Answer= At the replication fork:
Leading strand
Template is oriented 3 5
DNA polymerase synthesizes DNA continuously toward the fork
Lagging strand
Template is oriented 5 3
DNA polymerase cannot synthesize continuously
So DNA is made in short fragments called Okazaki fragments hence,
eplication is discontinuous
Role of enzymes involved
Helicase
Unwinds the double-stranded DNA
Primase
Synthesizes short RNA primers on the lagging strand
DNA polymerase III
Extends the primers to form Okazaki fragments (5 3)
DNA polymerase I
Removes RNA primers and replaces them with DNA
DNA ligase
Joins the Okazaki fragments into a continuous
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