PM-280 presentation

Assignment Brief PM-280 Journal Club Oral Presentation I need a 10-minute journal clubstyle oral presentation for the PM-280 Skills for Researchers module, based on the assigned research paper: CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex (Liu et al., Cell Reports, 2024). The presentation must critically analyse and clearly explain the paper for a scientific but non-expert audience, following the journal club format taught in the module workshops. Key Requirements 1. Structure (must follow CERN/CERI framework): Context: Background on hematopoietic stem cells, chromatin accessibility, pioneer transcription factors, and the BAF chromatin remodeling complex Why CUX1 is biologically and clinically important Experiments: Overview (not technical detail) of the main experimental approaches used Explanation of what each experiment measures and why it was used Results: Clear explanation of the main findings Interpretation of key figures (not just description) Linking chromatin accessibility to gene expression and lineage commitment Impact / Next Steps: Biological and clinical relevance Implications for hematopoiesis and cancer Limitations of the study and potential future research directions 2. Content Expectations: Demonstrate analysis and interpretation, not just summary Use specific evidence from the paper (figures, datasets, models) to support statements Explain complex ideas clearly and concisely Highlight the central narrative of the study Include a clear take-home message 3. Visual Material: Slides must be visually clear and well-organised Include key figures from the paper (not all panels; only relevant ones) Figures should be explained verbally and linked to conclusions Optional use of simple schematic diagrams (e.g. model of CUX1BAF recruitment) 4. Presentation Delivery Considerations: Content must fit within 10 minutes Slides should support spoken explanation rather than replace it Language should be scientific but accessible Presentation should be logically structured and easy to follow 5. Marking Criteria Alignment: The presentation must be designed to score highly in: Analysis and interpretation of data Use of evidence to support statements Background knowledge and wider relevance Structure, clarity, and organisation Quality of visual material Time management Ability to respond to questions 6. Final Output Needed: A complete slide-by-slide outline or finished presentation Clear indication of which figures to include and why Optional short speaker notes to guide verbal explanation